Analysis of Copper Iodide MTA Disk Inhibition of E. faecalis Biofilm Viability

M.S. Rotation project  with Dr. Michael Schmidt at the Medical University of South Carolina (MUSC)

MTA is the cement used by dentists when filling teeth. It is believed that the addition of copper iodide (CuI) nanoparticles to the cement would fortify the compound against bacteria known to promote tooth decay.

 

To test this concept in vitro, MTA disks were made with varying concentrations of CuI. E. faecalis  was cultured in LB broth. Disks were submerged in E. faecalis cultures and incubated. Each disk was then stained to determine the quantities of living and dead bacteria.

Bacterial Viability Protocol